Biodynamic Cosmetics

with OliPhenolia® - Demeter and Dermatest Certified

articles buttons

Research by RSC Pharma

The first step taken with Dr Schmidts and RSC Pharma was to begin a series of studies, using tests to verify the effects of OliPhenolia1 on human skin cells.
These tests (numbers 1, 2 and 4 where carried out in vitro on human keratinocyte cell lines, known as HaCaT, a consolidated method) regard four important aspects of the action that olive polyphenols have on skin:

1 - antioxidant
2 - anti-inflammatory
3 - anti-germ
4 - anti-age (regenerative)

Information on the individual studies will be published soon.


Oxidative stress is defined as a metabolic situation characterised by a high concentration of Reactive Oxygen Species, or ROS (the most common free radicals). ROS are harmful for the body due precisely to their reactivity. Oxidative stress accelerates ageing and inflammatory processes. High oxidative stress can damage cell structure; in order to defend itself the body need antioxidants.
Skin cells are continually exposed to ROS and therefore to oxidative stress, especially due to ultraviolet (UV) radiation which is the first cause of ageing and of skin cancer. When the quantity of ROS goes beyond the skin’s defence capacity, oxidative stress develops.

To verify the effects of OliPhenolia on oxidative stress in skin cells, cell lines of human keratinocytes, HaCaT, were tested with:
a) hydrogen peroxide (H2O2) to induce strong oxidative stress
b) DFCH-DA, a fluorescent molecule that reacts and activates itself in the presence of ROS and allows the quantity to be detected
c) OliPhenolia in different concentrations, 1:250 and 1:500
d) Vitamin C (250 µM), a very effective antioxidant, as a basis of comparison with OliPhenolia.

The experiments carried out had two different approaches:
A – Protective action: the cells were placed in contact with OliPhenolia (and Vitamin C) and then with H2O2.
B – Curative action: the cells were placed in contact with H2O2 and then with OliPhenolia (and Vitamin C).

It can clearly be seen that, in both cases, not only does OliPhenolia manage to reduce the action of free radicals – respectively by as much as 65% (graph 1A) and 63% (graph 1B) – but it is also decidedly more effective than Vitamin C.


After having induced inflammation (or rather an increase in the production of interleukin 8) in the cells, and verified a reaction to known anti-inflammatory active principles (such as hydro-cortisone), OliPhenolia was applied in three different concentrations in order to observe the reduction or modulation of interleukin 8 (IL-8) production.
After 72 hours (graph 2) the cells treated with OliPhenolia show a reduction of up to 78% (at a concentration of 1:200) in IL-8 production compared to a sample that was not treated, with a clear anti-inflammatory effect.


The antibacterial and antimycotic effects on various pathogenic agents were evaluated, in particular bacteria and mycosis causing skin disorders (for example acne).
The following pathogenic agents were analysed:
Bacteria - Staphylococcus epidermidis, Propionibacterium acnes, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa
Yeast - Candida albicans
Mould - Aspergillus brasiliensis

From the tests carried out, according to European pharmacopoeia standards (both suspension tests and diffusion tests on culture plaques), very noticeable antibacterial action and moderate antimycotic action can be deduced.


Cell renewal: natural regeneration as an anti-age programme.

Skin renewal is a continual process: the cells grow from the inside out, they keratinise and then fall off. As the years go by, the renewal process diminishes; cell division slows down. The tests carried out (in vitro) were aimed at verifying whether OliPhenolia was able to help skin regeneration.
Analysis of the proliferation behaviour of HaCaT cells (a cell line of human keratinocytes = skin cells) in the presence of OliPhenolia, show that after 24 hours the HaCaT cells, from an OliPhenolia concentration of 1:200 onwards, demonstrated a decidedly higher cell viability (cell division and renewal) compared to the samples that were not treated.

From the results shown in graph 4 it can be deduced that OliPhenolia has a positive impact on cell proliferation from a concentration of 1:200 onwards. The dilution of 1:100 did not show a positive effect, because excessive concentrations of polyphenols can irritate skin cells.
We can affirm that OliPhenolia has a “motivational” effect on skin cells. In the right quantity it helps cell renewal and skin regeneration. An adequate concentration favours – on average – regeneration by almost 40%.

1 In the biodynamic cosmetics OliPhenolia is used to refer solely to the purified extract of olive mill waste water (without grape juice or lemon juice).

© 2017 - Fattoria La Vialla di Gianni, Antonio e Bandino - Società Agricola Semplice
USt-IdNr.: IT01760910511 - Via di Meliciano, 26 - 52029 Castiglion Fibocchi (AR) - Toscana - Italia
tel. (0049-) 09471-301268 und (0049-) 08202-90008 - fax (0049-) 069-96758462 und 0039-0575-477704

E-Mail: fattoria@oliphenolia.it